FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3654022
Sequences flagged as poor quality0
Sequence length35-351
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG19800.060.1873663
CCGAGTA20800.060.094762
CACGATC8100.004686870657.97313320-329
GTAGCTG76750.056.611096
AGTAGCT76200.055.5724335
GCTGGGA150900.053.5176249
GAGTAGC49000.053.2456054
TACTCGG16750.052.496713
CTACTCG16550.052.449272
TAGCTGG87800.050.7927447
CCCGAGT26200.049.728041
AAGTGCT79100.049.5125735
TGCCCCG10700.00985648148.036156340-345
AAAGTGC84750.045.9386984
AGTGCTG82900.045.790246
CCAAAGT94500.045.499142
TTGGGAG106800.045.497426
CTCGGGA20650.045.1033825
GGAGGCT103700.044.6203169
GCTACTC41600.044.5427251
Produced by FastQC (version 0.11.5)