FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3654022
Sequences flagged as poor quality0
Sequence length35-251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18350.064.308723
CCGAGTA19150.062.2071462
ACGGATA3602.5606083E-561.811394245
AGTAGCT69750.061.0188375
AGACGAA7601.9131548E-458.558163245
TACTCGG19500.058.508513
GAGTAGC44950.058.2298434
CTACTCG19650.057.778062
GTAGCTG74200.056.3033526
GCTACTC46550.055.9972951
CCCGAGT23100.054.9686051
GCTGGGA149050.054.4818959
TAGCTGG82600.052.7442327
TTGGGAG115450.051.978456
GGAGGCT117850.050.2080849
AAGTGCT79350.049.548665
GCGAGTA2605.868927E-448.55062205-209
CGTACGC1200.00952834948.475155240-244
GCACTTT93950.047.3337751
AGTGCTG85650.046.0993586
Produced by FastQC (version 0.11.5)