FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2623618
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7450.046.1408043
CCGAGTA8050.044.477842
CTACTCG6400.040.4863552
GAGTAGC20100.039.8256724
TACTCGG6700.039.357893
TTCGGCG608.730436E-539.245
CCCGAGT10050.036.596931
GCTGGGA66250.036.5275239
GTAGCTG36450.035.8539126
AGTAGCT36900.035.6031235
TAGCTGG38100.035.5194637
GCTACTC16150.035.0368351
CCCAAAG48100.033.9193231
AAAGTGC38700.032.6087464
GGAGGCT46150.032.378329
CCAAAGT44300.031.903882
TTGGGAG48100.030.9815716
CGGAGGT10200.030.4048357
GGAGGTT29450.029.8370048
AAGTGCT41100.029.673465
Produced by FastQC (version 0.11.5)