FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2664233
Sequences flagged as poor quality0
Sequence length35-351
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGG855.907184E-4332.5053250-259
ACCGACC2700.002484738205.6979340-345
TAGCGTT1450.006025457153.06493300-309
CTCGATA1700.006898156146.28827320-329
CAACGAC4153.7105003E-4133.82756340-345
GCTAGTA7205.9472106E-497.479454310-319
CGCTACG1006.4844044E-495.47922210-219
GTAGACG2908.45738E-590.54064330-339
GATCGTT2650.001925581472.5782240-249
CCCGCGT1400.002364061368.93053300-309
TATAGCG1803.8476843E-565.2719310-319
GGAGCGA4505.0386006E-961.70937340-345
AGTGCGT3800.00603484354.396736290-299
AAGCCGG5100.00965860551.483894330-339
AACCGAC1650.00762194351.288597320-329
CCGTCAG4700.004849863249.77674310-319
CGAGTAG10900.049.6961823
CGTATAC2855.731966E-548.856323340-345
TCACGGA4805.01008E-448.739727310-319
GTAGCTG42300.048.4229436
Produced by FastQC (version 0.11.5)