FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3335811
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG3200.039.796641
ACGTCGA2200.034.3028261
CCGAGTA10450.024.7893582
CGAGTAG10700.024.1909053
CGCGAAT550.003063378221.36085738-39
GTCGAGA6000.021.19492
CCCGAGT14850.020.645221
GAGTAGC23900.019.8882564
GCTGGGA75150.019.0101689
AGTAGCT37450.018.4730575
CGCACTA1550.001019270318.2321722
CGAGACT13950.017.5817361
GTAGCTG40000.017.5102086
TAGCTGG45350.017.3053577
CCCAAAG52650.016.841941
TACTCGG7300.016.117233
CCAAAGT49450.015.7158012
TAGCGGG2104.3460034E-415.6646287
CTACTCG7350.015.379522
GGGAGGC55400.015.3535988
Produced by FastQC (version 0.11.5)