FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3335811
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGA550.002743190434.116919
CGAGTAG9250.024.866423
TACTCGG7400.024.1052043
CCGAGTA9650.023.3495962
CTACTCG7700.023.166392
GAGTAGC23600.020.8850864
GCTACTC22200.020.5803491
AGTAGCT37850.019.8426675
GCTGGGA75800.019.7421239
CCCGAGT13100.019.05631
GTAGCTG39250.018.5227646
TAGCTGG43300.018.4167867
GGAGGCT55900.018.0425959
TCGGGAG11250.017.9279146
CTCGGGA10600.017.7133225
CCCAAAG50200.017.2642651
TTGGGAG51650.016.8002266
AAAGTGC44550.016.648244
GGGAGGC66500.016.0126638
AAGTGCT45950.015.8340545
Produced by FastQC (version 0.11.5)