FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3266892
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11200.052.1205943
CCGAGTA12000.051.812942
CTACTCG9200.051.1985552
TACTCGG9200.050.658593
CCCGAGT14400.048.773351
GAGTAGC28600.046.0890464
AGTAGCT47750.045.3513455
GTAGCTG47750.044.595576
GCTGGGA95550.044.112589
GCTACTC24300.043.25711
TAGCTGG53200.042.131557
CCCAAAG62250.041.2682381
AAAGTGC51400.040.6654474
TTGGGAG72050.039.863386
CGGAGGT15900.039.010147
AAGTGCT54150.038.4263845
CCAAAGT57750.038.4162032
GGAGGCT71950.037.0952459
CAAAGTG60150.035.5325133
AGTGCTG58200.035.1344536
Produced by FastQC (version 0.11.5)