FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2982232
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG20100.073.1126253
CCGAGTA21550.071.111992
AGTAGCT74250.068.847645
GCGAAAA4100.00255818967.555954245
GAGTAGC47800.066.392714
GTAGCTG77350.065.868836
CTACTCG21100.065.5825352
CCCGAGT25550.064.03751
TACTCGG22000.063.6793563
GCTGGGA157400.063.4674079
AAGTGCT75950.061.2083635
TAGCTGG87950.060.7908677
GCTACTC48950.060.5391541
TTGGGAG118800.059.0596286
GGAGGCT126000.056.773989
AAAGTGC82200.056.5553934
GCACTTT91450.055.8007971
ACTTTGG91300.055.7409673
AGTGCTG83850.055.102446
CAAAGTG87750.053.825523
Produced by FastQC (version 0.11.5)