FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3081116
Sequences flagged as poor quality0
Sequence length35-351
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAC2150.001096932583.640076340-345
CGAGTAG26450.080.1441353
CCGAGTA27700.079.234592
AGTAGCT91800.074.2713555
ACCGCAT4850.001779339674.027145340-345
GTAGCTG93650.071.07336
GAGTAGC62150.070.210634
CCCGAGT32800.068.8283161
GCTGGGA182200.065.462289
TAGCTGG105600.065.00187
AAGTGCT84100.062.940945
TTGGGAG116950.061.784066
AGTGCTG91250.057.6755646
CGACTAA2054.454021E-556.672035330-339
TTTGGGA108650.056.135395
GCACTTT97200.055.1936951
GGAGGCT121700.054.317339
CAAGTAG38400.054.0735553
AAAGTGC97500.053.5277254
ACTTTGG103150.052.698923
Produced by FastQC (version 0.11.5)