FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3081116
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG3251.2471875E-5101.59684245
CGAGTAG24350.077.113043
CCGAGTA25950.076.022192
AGTAGCT85500.074.641075
GTAGCTG88300.071.7376
GAGTAGC56950.071.680774
TTGGGAG134050.065.561386
CTACTCG25000.064.8876952
CCCGAGT30200.064.74711
GCTGGGA182300.064.701389
AAGTGCT88450.063.5525475
TAGCTGG101700.063.3950737
TACTCGG25150.063.083093
GGAGGCT145400.060.5652439
GCACTTT100950.060.287181
AGTGCTG92500.060.2570466
ACTTTGG103950.059.163733
TTTGGGA116200.058.909595
TCGATAG1350.004530240358.498802240-244
CAAGTAG35250.058.4938243
Produced by FastQC (version 0.11.5)