FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944201
Sequences flagged as poor quality0
Sequence length35-351
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAC3953.1453348E-4114.47045310-319
CTACGAA3400.001566956276.44311340-345
AGTAGCT72800.066.951045
CGAGTAG20100.066.8838043
GAGTAGC46100.065.645344
GTAGCTG75450.063.9255876
CCGAGTA21550.062.6830752
GACCTAA8200.003823907361.01898330-339
TAGCTGG83450.059.6290557
GCTGGGA152550.058.7218789
CTACTCG16650.057.0662572
CCGGTTA2257.221135E-656.208145280-289
CCCGAGT24300.056.0947651
CGTATCC1900.00544883255.830036340-345
TACTCGG17200.054.8788073
AAGTGCT74450.054.7865565
GCTACTC38100.052.4628751
TTGGGAG94950.052.0008286
AGTGCTG78700.051.9911966
AAAGTGC80150.049.6769454
Produced by FastQC (version 0.11.5)