FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643294
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG25350.068.813813
AGTAGCT92350.067.881465
GAGTAGC60050.066.824414
CCGAGTA26750.065.01142
GTAGCTG94700.064.3625646
TAGCTGG102100.060.3394667
GCTGGGA180150.059.1023839
CCCGAGT31550.055.938611
ACGACTA4101.3726029E-553.663807340-345
TTGGGAG122500.051.3142246
AAGTGCT89700.051.1266865
GGAGGCT118450.049.2048659
CTACTCG22500.048.7867242
TACTCGG22500.048.490643
AGTGCTG95300.047.9357076
CAAGTAG40700.047.404233
GATTCGC2855.630584E-446.103188340-345
TGAGTAG46650.045.7184643
AAGTAGC44350.045.5876664
CCAAGTA43750.045.38812
Produced by FastQC (version 0.11.5)