FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643294
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT86650.065.684055
GTAGCTG87300.064.856336
CGAGTAG24300.063.4325833
CCGAGTA25050.062.952552
GAGTAGC57000.062.1981774
GCTGGGA176950.062.11539
CTACTCG24000.060.7663652
TACTCGG23750.060.158753
TTGGGAG134450.059.221376
GCTACTC52750.058.9038431
TAGCTGG100150.058.6643147
AAGTGCT89550.058.4591375
GGAGGCT137150.055.9342589
CCCGAGT30600.054.84031
AAAGTGC97200.054.162414
GCACTTT108550.052.3870051
TCGGGAG30850.052.2729456
AGTGCTG98200.052.223736
TTTGGGA127300.052.161125
ACTTTGG111450.051.5452963
Produced by FastQC (version 0.11.5)