FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3254810
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8050.042.6268583
CTACTCG7600.041.4429742
CGAGTAG6900.040.875073
GCTACTC21700.037.709681
GCTGGGA69900.036.0426989
CCGAGTA8250.035.8985022
CGGAGGT12150.035.5910847
GGAGGCT54300.035.230949
CCCGAGT10550.035.215831
GTAGCTG37200.034.3681076
GAGTAGC22000.034.1864244
AGTAGCT36850.034.185375
CTCGGGA10500.034.0225835
TAGCTGG40050.033.800237
TTGGGAG54500.033.2905126
CTCGCGT1001.4107318E-632.9044344
GGCGGAG17200.032.247545
TCGGGAG11050.031.0520486
CCCAAAG54200.030.9764921
GCGGAGG15050.030.9192076
Produced by FastQC (version 0.11.5)