FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293364
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7650.049.239143
CCGAGTA7900.047.703872
GAGTAGC21350.046.0925064
GTAGCTG33950.043.479026
AGTAGCT34900.042.497865
GCTGGGA65650.041.0119939
CTACTCG5800.040.6099782
TAGCTGG36600.040.0557147
CCCGAGT9950.039.796171
GCTACTC15650.038.5551
TACTCGG6300.038.116373
TTGGGAG48350.037.23856
CCCAAAG42300.036.6640661
GGAGGCT47250.036.1984989
TCGGGAG8450.035.048846
AAAGTGC35950.034.9698944
CGGAGGT11750.033.5917327
GGGAGGC61300.033.574128
AAGTGCT38050.033.4111185
AGTGCTG38700.033.2835356
Produced by FastQC (version 0.11.5)