FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671114
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG657.185008E-1058.0179941
GCTGGGA45600.032.6678669
AACGCGT452.5986048E-531.32817580-81
CCCGAGT9500.030.7648071
CCGAGTA7250.030.5365432
GAGTAGC16650.029.9742264
TACTCGG5100.029.5417443
TAGCTGG28000.028.6988457
GGAGGCT35850.028.4444089
CGAGTAG7000.028.2492923
CTACTCG5350.028.1745012
CCCAAAG40450.027.7360461
AGTAGCT27000.027.3774015
GTAGCTG27650.027.0292516
TTGGGAG37750.026.6458036
CGCGTAG459.531395E-426.10681282-83
GCGTACA909.530143E-426.1068127
GGGAGGC40200.025.8340518
GCCCGTA1101.0199718E-425.6321439
GTGCTGG31200.025.3035247
Produced by FastQC (version 0.11.5)