FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671114
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA6900.036.106332
CTACTCG5900.035.0555882
CGAGTAG7000.034.9196623
GGAGGCT42000.034.2468459
GCTACTC16200.032.8018951
TACTCGG6550.032.2950483
CCCGAGT9250.032.0283361
GCTGGGA48550.031.6597149
CGCGAGT1501.0641088E-931.3504871
CGGAGGT11100.031.3375727
TTGGGAG43550.029.8984176
GGCGGAG14100.029.670685
AGGCGGA14750.029.638464
GAGTAGC16050.028.9951154
CCCAAAG38300.028.8539071
TAGCTGG28000.028.3716957
TCGGGAG8700.028.0957536
GCGGAGG12550.028.0914486
GGAGGTT27950.027.9179088
CCGAACG856.7966885E-427.6621931
Produced by FastQC (version 0.11.5)