FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3656826
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA901.3096724E-841.7975394
TACTCGG9000.041.264333
CGAGTAG8600.040.9970933
CTACTCG8900.039.0858962
CCGAGTA10700.037.3433722
CCCGAGT13750.034.8843231
GAGTAGC22800.032.5771064
GCTGGGA74200.032.120519
CGGAGGT13050.030.9806187
GGAGGCT58800.030.379789
GGCGGAG17950.030.118655
CTCGGGA12350.029.6913365
GTAGCTG40750.029.5334686
AGTAGCT39550.029.4786325
TCGGGAG13650.029.2744276
TTGGGAG57650.028.9488416
TAGCTGG43400.028.7050487
AGGCGGA19200.028.4018754
CCCAAAG58850.028.0474591
GGGAGGC73000.027.6275588
Produced by FastQC (version 0.11.5)