FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415416
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT555.22917E-542.804025
CGTCGAG459.989166E-441.9067081
GCCCGTA608.837099E-539.1590429
CTACTCG4250.032.144192
GAGTAGC12550.031.8898454
GCTGGGA45300.031.5346989
CGATACG600.00411544531.4052492
TACTCGG4550.031.0453153
AGTAGCT23500.029.8535255
GTAGCTG23800.029.428396
GGAGGCT30300.029.0009569
CCGAGTA5600.028.6012042
CCCGAGT7300.028.4161911
TAGCTGG26500.027.8398637
CGAGTAG4950.027.5853823
CCCAAAG35450.026.9970231
TTGGGAG34200.026.939356
GCTACTC12150.026.7737311
GGGAGGC35250.026.6614768
CGCGTTG909.5131376E-426.1146776
Produced by FastQC (version 0.11.5)