FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415416
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG5750.040.8800663
CCGAGTA5750.040.879222
CGATACG608.8147004E-539.175922
CTACTCG5300.038.1410832
CCCGAGT7150.036.836671
TACTCGG5750.036.7920573
CGGAGGT8450.033.9356047
GCTACTC14050.033.475111
GCGGAGG8850.032.932976
GAGTAGC13450.032.506494
GCTGGGA46500.032.04719
GGCGGAG11150.031.6211625
GGAGGCT37550.031.1725149
AGGCGGA12700.030.3542524
TTGGGAG38050.030.2687056
AGTAGCT25000.029.1462785
GGAGGCG12900.028.4253182
CGAATCG505.33122E-528.19673336-37
GGAGGTT24450.028.0709068
GTAGCTG25550.027.9663076
Produced by FastQC (version 0.11.5)