FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2849331
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT1201.8189894E-1243.163184
TACTCGG7200.040.5472263
CTACTCG6900.039.5992662
TCGCGTT1101.7662387E-938.525815
GCTACTC15950.037.827681
CCGAGTA7500.037.6875762
CGAGTAG7900.035.7623563
GCTGGGA56000.033.816929
AGTAGCT31700.032.0845835
CTCGGGA9950.031.2336545
TAGCTGG34600.030.5578947
GAGTAGC18400.030.1971654
ACTCGGG10450.030.1898164
GTAGCTG32550.030.1874036
TCGGGAG10200.029.9601276
GGAGGCT45300.029.46039
CGGAGGT10100.028.8461177
CCCGAGT10250.028.5120351
CCCAAAG43950.028.0997711
TTGGGAG45350.028.0945766
Produced by FastQC (version 0.11.5)