FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2789195
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACTC16600.036.546061
CGGAGGT10000.035.2591747
TACTCGG6700.034.3826943
CGAGTAG6450.034.257593
GGAGGCT46000.034.0303279
CTACTCG6250.033.84822
CCGAGTA7050.033.341412
GCTGGGA55800.032.93999
TTGGGAG47700.031.1443716
CCCGAGT9150.030.8382031
AGTAGCT30150.030.2505345
TAGCTGG31700.029.9573217
GTAGCTG29850.029.766546
GCGGAGG11550.029.713366
GAGTAGC16650.029.0826424
CCCAAAG44650.028.8594471
GGGAGGC56700.028.273678
GTGCTGG34400.028.1526747
GGCGGAG12350.027.7891085
TCGGGAG8800.027.7799556
Produced by FastQC (version 0.11.5)