FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2516576
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT657.366907E-1057.8577885
CTACTCG6500.046.2880672
TACTCGG7050.042.6769453
CGAGTAG8400.041.4147343
CCGAGTA8500.040.92752
CGGAGGT10350.038.606037
GAGTAGC19200.037.9510964
AGTAGCT30550.037.8537645
CCCGAGT10200.037.8076931
TTGGGAG45400.037.793196
GGAGGCT46750.037.3036739
GCTACTC17150.036.7457541
GCTGGGA56450.036.3895579
TAGCTGG35200.035.256397
GTAGCTG32550.035.238346
TCGGGAG10900.033.6391226
GGGAGGC60850.033.064338
GCGGAGG12300.032.8677446
GGCGGAG14100.032.6732375
CTCGGGA10350.032.248035
Produced by FastQC (version 0.11.5)