FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4024084
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG10050.040.6950532
TACTCGG10600.039.9164623
CGAGTAG9750.035.199273
CCGAGTA10550.034.756062
GCTACTC25000.032.734691
TCGGGAG13350.032.045336
GAGTAGC25950.031.3418144
CTCGGGA13350.031.3414255
CCCGAGT14450.031.2465721
GGAGGCT65000.030.302499
TTGGGAG62100.029.2213526
GCTGGGA81150.029.1378219
CGGAGGT15200.028.763657
AGTAGCT41150.028.4471995
GTAGCTG41500.027.8670926
GGGAGGC80950.027.5858768
GGCGGAG21400.027.0209965
CCCAAAG64900.026.8136561
TAGCTGG47200.026.49387
ACTCGGG16000.026.4446584
Produced by FastQC (version 0.11.5)