FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647557
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG6750.043.9755062
TACTCGG7150.043.470033
CGAGTAG7650.041.2444463
CCGAGTA8650.039.2184562
GCTACTC17000.039.11241
AGTAGCT33700.038.0094035
GCTGGGA66950.037.9733739
CGGAGGT10850.037.6802527
GAGTAGC20100.037.4865954
CCCAAAG47150.035.605131
GTAGCTG35800.035.4583366
TAGCTGG37250.035.3228767
AAAGTGC35950.035.237534
CCCGAGT10750.034.2160951
GGCGGAG14350.034.1297845
CTCGGGA9450.033.8866925
AAGTGCT38250.033.6111455
AGGCGGA13600.033.588064
TTGGGAG48250.033.4221536
GGAGGCT47400.033.3114439
Produced by FastQC (version 0.11.5)