FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177117
Sequences flagged as poor quality0
Sequence length35-351
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATATA1500.009013952133.78372310-319
CTAGCCG1601.4690548E-5125.03139310-319
CCTTACG3000.001781973676.87539340-345
CGAGTAG16050.061.3730553
GTAGCTG59800.057.9117856
AGTAGCT61400.057.3739665
CCGTACC3756.997696E-456.31398320-329
CCGAGTA17600.056.3046572
GAGTAGC39550.053.752224
GCGCTAG2000.001857315552.88925320-329
TAGCTGG67650.052.6517877
GCGCATA2200.007343644351.77253300-309
GCTGGGA122150.051.5872659
CCCGAGT21400.049.7238581
CTACGAG3550.006312538449.141106290-299
CACACGA7150.00951059848.478104340-345
TTGGGAG82500.046.42436
ATGCGTG4350.002438631145.988556310-319
AAGTGCT66600.044.371065
CGAATAG3650.007780506344.03123280-289
Produced by FastQC (version 0.11.5)