FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177117
Sequences flagged as poor quality0
Sequence length35-251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA2559.232904E-5155.74681245
CACGCAA4458.4653177E-489.24817245
CGCGTAG1200.002169685775.8927235-239
CGCTACG1000.002699022966.66921230-234
CGAGTAG16250.059.258353
CGCTAAG2300.004787392457.68384230-234
TAGGTCG2302.8493977E-457.558605245
GAGTAGC37950.057.4860234
GTAGCTG61350.055.4227756
AGTAGCT62950.055.3977285
CCGAGTA17800.055.31992
GACCGGT2450.0082437754.03461245
CTACTCG16050.053.8945542
TACTCGG17350.052.99283
GCTGGGA121350.052.2253769
TAGCTGG68100.052.165347
GCTACTC39400.049.582731
TTGGGAG101100.049.2909436
CCCGAGT21050.048.341681
GGAGGCT95650.047.830679
Produced by FastQC (version 0.11.5)