FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3256146
Sequences flagged as poor quality0
Sequence length35-351
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAG2201.719394E-484.14282320-329
CAACGCG1600.001557056776.58167340-345
CGAGTAG23000.067.644013
AGTAGCT79200.064.5438845
GAGTAGC52100.063.2425654
CCGAGTA25400.062.380782
GTAGCTG81550.062.2791146
TAGCCCG2502.2967652E-558.82669280-289
CTACTCG16600.057.6049772
TAGCTGG89650.057.312927
GCTGGGA159350.056.9669659
TACTCGG17850.056.3429373
AAGTGCT77250.054.9524845
CCCGAGT29650.052.919821
GCTACTC38100.051.8438571
CCAAAGT91600.050.011672
TTGGGAG109000.049.5486266
AGTGCTG85800.049.1020626
AAAGTGC86550.048.790924
TACGCTG4550.00941415148.61214280-289
Produced by FastQC (version 0.11.5)