FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3256146
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGT1100.0054998216157.82965235-239
CGGTACG656.9224194E-493.9289220-224
GTCTAGT9602.597335E-885.23519245
CGAGTAG17850.067.919143
AGTAGCT72850.065.9719245
CCGAGTA18650.065.879372
GAGTAGC47400.064.741164
CGCGGTA1050.003491939362.47406235-239
GTAGCTG76150.062.2591636
TAGCTGG82700.059.5522657
CTACTCG21250.058.5829622
TACTCGG21700.058.1137123
GCTGGGA163300.057.631849
AAGTGCT79900.057.4413765
TTGGGAG118900.056.533886
CCCGAGT23200.056.2460061
AAAGTGC84800.053.9307674
GGAGGCT125900.053.9039849
GTCGATA1650.001624272253.627457240-244
GCACTTT95850.052.479081
Produced by FastQC (version 0.11.5)