FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461776
Sequences flagged as poor quality0
Sequence length35-351
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTA200.00423232371390.1743290-299
CGCTAAC753.9505248E-4380.27075220-229
CCAACCG2257.612423E-4305.2548320-329
CAACGAC3000.0012945778255.61713340-345
CTACGCT1400.002732733199.34253290-299
TAATCCG1850.0028084011197.4987330-339
CTCGTAC2350.0036416485181.05138260-269
TTCGCGT800.004143914173.49345320-329
TCGCGTT750.005843016154.6706320-329
CGCGAAA300.007282881143.70514140-149
CAACCGA2450.0077864127140.4463320-329
ACCACGT5650.009960292129.24461330-339
CCGAAAG3500.008268437109.550186340-345
AAACGAC2706.296979E-496.154236320-329
CGACATA1659.848389E-485.93805290-299
CGGCTTT4600.00597537183.35341340-345
CTCGCAA2100.00358305476.93705310-319
CAACGCA5150.001727459174.561554340-345
ACGCAAC1800.001887129772.95353320-329
CGAAATC4205.605219E-471.55591300-309
Produced by FastQC (version 0.11.5)