FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461776
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGG1500.0010847261271.35413245
TCGATAG1450.0029341017194.65936240-244
TCGACGG1100.004285918185.01418245
TAGCCCG1450.00868136135.46384190-194
GTCTAGT8601.7622453E-594.65842245
CGATAGC1157.126627E-493.23302240-244
ACGGCAA3850.00112656383.04876200-204
CGACGAC1909.808611E-472.21133235-239
ATAGTCG1200.002540903667.68811200-204
AAGCGTA3054.0313935E-566.726425245
CGGTAGT2200.005375585564.14911240-244
CCGCAAT1950.003318580863.273003220-224
GGGTACG2100.0036987960.54009225-229
ACGCAGT3600.004242645659.457813155-159
ATCCCCG2558.523341E-455.344334240-244
TCCGGTA2251.6700287E-454.45233220-224
ACGTTTA3800.006042435354.3767235-239
CCGAGTA9050.053.548742
GCTTCCG5158.9810237E-753.282146235-239
ATAGCGG2354.659314E-452.135212220-224
Produced by FastQC (version 0.11.5)