FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3667184
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG4352.3483153E-9120.52133245
CGAGTAG24150.072.2463
AGTAGCT90150.070.317375
CCGAGTA25550.069.001832
GTAGCTG92500.067.64836
GAGTAGC60050.067.259284
CGCCGAA2650.002619698667.16586240-244
CTACTCG26300.063.590562
GCTGGGA188500.062.2763069
CCCGAGT30000.060.7419621
TAGCTGG106450.060.209347
TTGGGAG148000.059.526596
TACTCGG28000.059.5092053
AAGTGCT99050.058.452515
GCTACTC60000.058.1185571
GGAGGCT152200.056.8655329
AGTGCTG106900.053.2861256
TTTGGGA130300.052.6811265
AAAGTGC110500.052.4503064
GCACTTT116200.052.4125671
Produced by FastQC (version 0.11.5)