FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3178698
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT804.5856723E-947.0679055
CGAGTAG7850.038.373833
CTCGCGT1003.2880052E-837.6543244
CCGAGTA8300.036.8917922
TACTCGG6450.032.1083373
ACTCGCG1201.6330341E-731.3786053
CGCGTTG1201.6500417E-731.3415726
CCCGAGT12800.030.958551
CGGAGGT8750.030.6126657
GCTGGGA56600.030.1387029
GAGTAGC19100.030.0643144
CTACTCG6500.029.7142682
TAGCTGG34650.028.345067
GCTACTC15450.028.0912191
AGTAGCT32350.028.0806965
AGGCGGA12400.027.329754
GTAGCTG33650.027.1037046
GGAGGCT42500.026.9795849
GGCGGAG12300.026.4039465
CTCGGGA7850.025.1828255
Produced by FastQC (version 0.11.5)