FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3235739
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA702.1100281E-433.7738994
CTACTCG7050.033.29732
TACTCGG6700.031.531093
CTCGCGT1201.6705417E-731.2975274
TCGCGTT753.29889E-431.2975235
CGCGAGT1102.9172443E-629.978781
GCTACTC15000.029.20791
CGACCGT650.00620947828.8717148
AGGCGGA12150.028.2064134
CGGAGGT8800.027.7229797
GGCGGAG12850.026.6698785
CTCGGGA8300.026.5840445
GCGGAGG11100.024.932226
TCGGGAG10600.024.3382176
GGAGGCT39800.024.1666729
GGAGGCG14050.023.7252162
CCCAAAG43650.023.2039991
GCTGGGA47350.022.9886829
GGGAGGC45550.022.1450168
TACCGGG1303.1858875E-421.6675195
Produced by FastQC (version 0.11.5)