FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3006411
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA9750.049.2920952
CCCGAGT11650.048.9725461
CGAGTAG9450.048.8299643
TTCGGCG652.7515944E-643.464035
GAGTAGC23700.041.920484
AGTAGCT36400.040.2301035
GCTGGGA73850.038.2407849
TACTCGG7100.037.8014643
GTAGCTG37700.037.656666
CCCAAAG52100.036.3815841
GCTACTC19650.035.5134121
TAGCTGG42900.035.37927
CTACTCG7600.035.338082
GGAGGCT50950.035.04639
AAAGTGC41700.034.2135964
TTGGGAG55200.031.7647866
AAGTGCT45200.031.4601385
TCGCGTT1052.0375483E-631.390695
CGGAGGT10600.031.0309437
GTGCTGG46300.030.751377
Produced by FastQC (version 0.11.5)