FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3006411
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG9050.052.986583
CCGAGTA9700.051.370332
CTACTCG8250.049.0030022
TACTCGG9150.045.214313
GAGTAGC23700.043.6403924
TCGCGAA450.001012133841.79524294
CCCGAGT13000.041.2484051
GCTGGGA77550.040.858089
GCTACTC22250.040.3783871
AGTAGCT40050.040.1442575
GTAGCTG40650.039.3204276
TAGCTGG42750.039.1483737
CTCGGGA11350.038.1058655
CCCAAAG52450.037.307271
CGGAGGT14000.035.5940747
TCGGGAG12550.035.2114566
TTGGGAG58800.035.178336
GGAGGCT60050.034.915789
AAAGTGC43250.034.7838944
AGGCGGA20650.034.377264
Produced by FastQC (version 0.11.5)