FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3410956
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCC4650.001228295681.27485340-345
AGTAGCT89200.067.346845
GTAGCTG91050.066.602356
CGAGTAG26300.066.1519243
CCGAGTA28450.062.2861862
GAGTAGC61650.062.166974
TAGCTGG100050.061.8508837
GCTGGGA174500.061.3285879
TTGGGAG118200.056.7381746
CCCGAGT32050.056.1066361
AAGTGCT86550.054.041335
TTTGGGA113350.050.9961055
GGAGGCT115900.050.9482089
AGTGCTG94500.049.6441046
GCACTTT102150.049.2903441
AAAGTGC96700.048.1081964
GTGCTGG99650.046.874447
ACTTTGG108950.046.5755353
GGGAGGC160500.046.5177968
CAAGTAG38850.046.2426833
Produced by FastQC (version 0.11.5)