FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3410956
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG22850.071.0493243
AGTAGCT83000.069.919585
CCGAGTA23950.068.294972
GTAGCTG86100.067.333776
GAGTAGC53550.065.94994
CTACTCG22800.062.7057882
TTGGGAG128300.061.948576
GCTGGGA169750.061.274299
TAGCTGG98950.060.0588957
CCCGAGT27600.059.717961
TACTCGG24100.059.572073
GGAGGCT131900.058.41989
GCTACTC54200.057.242131
AAGTGCT86500.056.5156635
TTTGGGA116950.055.2682345
GCACTTT105250.054.6373061
ACTTTGG107300.053.8027653
AGTGCTG90800.053.4405336
CTCGGGA28600.052.740555
CACTTTG110650.051.9023972
Produced by FastQC (version 0.11.5)