FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3170522
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7500.035.0994722
TACTCGG7850.033.5355833
CGGAGGT11250.033.0096937
GGCGGAG13400.032.6261375
GGAGGCG13300.031.1031822
AGGCGGA13650.030.9954094
GCTGGGA61200.030.9542739
CTCGGGA10000.030.086215
GCTACTC18950.030.0295411
GCGGAGG12800.029.3796756
GGAGGCT46050.029.2967369
TCGGGAG11450.027.9171056
GGGAGGC58200.027.865268
GAGTAGC18750.027.5790254
CGAGTAG7500.026.9522293
CCGAGTA8050.026.8618412
CCCGAGT11050.026.3877261
AGTAGCT33050.026.3140565
TTGGGAG48250.026.2072776
GTAGCTG33750.025.7670636
Produced by FastQC (version 0.11.5)