FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2984201
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG11350.055.9184653
CTACTCG11700.055.4474372
CGAGTAG10700.050.0885433
GCTACTC27300.048.5815431
CCGAGTA11800.045.416232
AGTAGCT42350.045.178995
CTCGGGA14700.044.452175
GAGTAGC25550.044.1601374
AAAGTGC51700.043.2839054
TCGGGAG16600.043.044386
CCCAAAG62250.042.686831
GGAGGCT74900.042.6141979
ACTCGGG15250.041.9255874
GTAGCTG46050.041.853836
TTGGGAG68900.041.8236736
GCTGGGA94100.041.712219
AAGTGCT53650.041.0959135
TAGCTGG48300.040.9747127
CCAAAGT59000.040.635572
CCCGAGT14300.039.795521
Produced by FastQC (version 0.11.5)