FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3992545
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA1950.045.9611131
CGTCGAG3500.035.041031
CGAGTAG12100.023.3381673
CCGAGTA12550.022.51972
GAGTAGC27400.021.2996274
GCTGGGA82050.021.019339
GCTACTC22400.020.8477291
TACTCGG9400.020.0277883
AGTAGCT46600.019.593715
CTACTCG9200.019.455882
GTAGCTG46600.019.3705776
CCCAAAG63300.019.151421
CCCGAGT18000.017.8200111
TAGCTGG53300.017.8096567
GTCGAGA6850.017.1911332
CCCGTAT2756.3624066E-717.1529521
GTGCTGG53000.016.4031497
CCAAAGT63700.015.7505322
TCGGGAG14650.015.724826
CTCGTAT11550.015.4637494
Produced by FastQC (version 0.11.5)