FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3638226
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT906.121245E-736.6116525
CGCGATT550.002664626634.319791
CGTCGAG1651.8189894E-1234.319791
CTACTCG5600.032.8126372
TACTCGG6450.032.111223
CTCGCGT1501.0531949E-931.381424
GCTGGGA60800.029.216179
CGGAGGT9000.027.6737757
TCGCGGG856.824712E-427.6430637
GCTACTC17600.026.0079651
TCGGGAG8500.025.9955546
CGAGTAG7050.025.3722113
CCGAGTA7300.025.1713392
GGAGGCT46300.025.1713099
GAGTAGC20200.025.1672784
ACTCGCG1501.1482425E-625.1051353
AGTAGCT36750.024.9770475
GGGAGGC48500.024.8046638
CTCGGGA8000.024.7128685
TAGCTGG39100.024.3980087
Produced by FastQC (version 0.11.5)