FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3638226
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT602.5873305E-854.7542885
TACTCGG6900.038.770123
CTACTCG7650.037.4225852
CTCGCGT1151.1529846E-732.6485184
GCTACTC19500.032.6081621
TCGGGAG10400.030.2164966
CGAGTAG6850.030.146263
CTCGGGA9000.029.7237555
GCTGGGA64700.029.655099
GGAGGCT52400.028.8273569
GGGAGGC61750.027.044658
CGGAGGT10700.026.3048487
GGCGGAG15550.026.2579145
AGGCGGA16100.026.2354184
TTGGGAG51150.025.9503656
GAGTAGC19200.025.666074
ACTCGCG1101.0289684E-425.5994073
CCGAGTA7950.025.384392
CCCAAAG54750.025.1203651
AGTAGCT33550.024.3404185
Produced by FastQC (version 0.11.5)