FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4271946
Sequences flagged as poor quality0
Sequence length35-351
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT134850.079.985655
GTAGCTG136850.077.831346
CGAGTAG39650.077.804133
CCGAGTA42600.073.852772
GAGTAGC96050.070.3018654
GCTGGGA283400.069.6375669
TAGCTGG159000.068.654667
TTGGGAG184800.065.8356256
AAGTGCT137450.065.321415
CTACTCG34400.060.8266532
CCCGAGT51750.060.4323771
AGTGCTG149850.059.7224736
TACTCGG35800.058.8385433
GGAGGCT193100.058.2302559
TTTGGGA170050.057.762365
TCGGGAG41950.056.2251326
CAAGTAG63000.055.251133
GTGCTGG162850.055.1647347
AAGTAGC66100.054.2419934
GGGAGGC269750.053.937518
Produced by FastQC (version 0.11.5)