FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3648839
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1305.456968E-1239.9071541
ACGTCGA1101.7389539E-938.587911
TACTCGG7200.028.1130523
CTCGCGT856.757352E-427.690014
CTACTCG7200.027.4769882
CGAGTAG8300.027.2229483
GCTGGGA62700.027.1319149
CGGAGGT9800.026.8535297
CCGAGTA8750.025.3012562
GAGTAGC21700.025.1634564
AGGCGGA12900.024.8136844
GCTACTC19550.024.6067851
GGAGGCT51600.024.5897149
AGTAGCT36550.024.4702425
GGGAGGC56750.024.1800068
TTGGGAG51450.024.0322466
TAGCTGG39750.023.2899487
TCGGGAG9750.023.145186
GTAGCTG36700.023.0584646
CCCGAAT6350.023.0244561
Produced by FastQC (version 0.11.5)