FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3648839
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7750.032.705363
CCGAGTA8200.032.0562672
TACTCGG7800.030.6903953
GCTGGGA66850.030.3819479
GGAGGCT51800.030.1539069
GAGTAGC22900.029.3111884
CGGAGGT13750.028.6537787
CTACTCG7950.027.750422
GCTACTC21400.027.07361
AGTAGCT37300.026.6776755
GGCGGAG16500.026.4557445
TAGCTGG41400.026.284097
TTGGGAG54350.026.2323786
AGGCGGA17500.026.017564
GTAGCTG38150.025.93896
GCGGAGG14950.025.723876
GGGAGGC67100.024.9553058
GGACCGA3050.024.6057918
CGCGAGT1351.4338892E-524.4241221
TGGGAGG72850.023.6931937
Produced by FastQC (version 0.11.5)