FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3625004
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10400.040.7308963
CCGAGTA10600.040.43772
CGTCGAG952.0534571E-839.7202641
CTCGCGT1209.640644E-1139.2223434
GAGTAGC26000.038.1965334
CTACTCG7600.037.1867832
CCCGAGT13600.037.109971
TACTCGG7700.035.4529233
GCTGGGA79000.035.334329
TCGCGTT959.3536255E-734.6808135
AGTAGCT41550.034.0965355
GTAGCTG42650.033.8358046
TAGCTGG44350.033.8013847
CGGAGGT11050.032.7465747
GCTACTC18700.031.5292871
CCCAAAG59450.030.1493191
TTGGGAG57450.030.110296
GGAGGCT53850.029.1472879
GGGAGGC65650.028.6327238
AAAGTGC52300.027.7181244
Produced by FastQC (version 0.11.5)