FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3487468
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGCG1055.05388E-835.8602185
CGCACTA1001.4123216E-632.89980794
CCGAGTA9550.030.0906642
CGAGTAG9150.029.8345263
CGCGAGA804.6835007E-429.4805071
TACTCGG6600.029.23833
TCGCGTT650.00611346528.9640225
CCCGAGT13150.028.6958541
GAGTAGC20500.028.239924
CTACTCG6850.028.196722
GCTGGGA56050.027.667599
TCTCGCG856.8037066E-427.6576046
CGGAGGT9400.027.4958937
TAGCTGG35650.026.099897
AGTAGCT32850.025.9331575
GCTACTC16900.025.6776961
GTAGCTG34200.024.8837556
GGGAGGC52500.024.6153728
TTGGGAG48900.024.1339456
GGAGGCT47800.023.8897369
Produced by FastQC (version 0.11.5)