FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3706321
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA10950.048.4472962
CGAGTAG10300.047.8589483
CCCGAGT13450.041.6924671
CTACTCG10050.039.7061772
GCTGGGA84950.039.321379
TACTCGG9700.039.2034533
GAGTAGC27400.037.8668024
GCTACTC25450.036.2911951
GGAGGCT65300.035.9945349
AGTAGCT45050.035.848815
GTAGCTG44150.035.483166
TAGCTGG48450.034.759827
TTGGGAG64850.033.9933856
AAAGTGC54650.033.1599664
GTGCTGG55600.032.5678757
CCCAAAG64750.032.021811
AGTGCTG57950.031.403996
GGGAGGC80550.031.3910568
AAGTGCT58950.031.0593155
TGCTGGG62200.030.9980348
Produced by FastQC (version 0.11.5)