FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3642788
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9500.043.9713482
TACTCGG10050.040.6303563
GCTACTC23150.039.8784981
CCGAGTA11550.037.386022
GCTGGGA81150.036.7618689
CGAGTAG11250.036.713653
TTGGGAG62000.035.779386
GAGTAGC26050.035.4945684
CCCGAGT14650.034.7232061
GGAGGCT66600.034.369619
AGTAGCT40550.033.7969475
CGGAGGT14150.033.4788137
GTAGCTG40200.033.132616
CCCAAAG64200.032.868261
CTCGGGA12650.032.2785645
TAGCTGG46100.031.9472777
AAAGTGC52000.031.5913434
TCGGGAG14300.031.484636
AAGTGCT53850.030.6790875
GTGCTGG52950.030.6489287
Produced by FastQC (version 0.11.5)